NON DOMESTIC SERUM PLASMA
Human AB SERUM PLASMA is used in tissue engineering, transplantation, and cell therapy applications for the expansion of mesenchymal stem cells (MSC) from adipose tissue or mesenchymal stromal cells from human bone marrow; for the standardized generation and transplantation of limbal epithelial stem cell grafts; for ex vivo expansion of peripheral blood NK cells in hematopoietic stem cell expansion medium; and to improve human islet culture technology prior to transplantation.
Warning
Each donor is screened for antibodies to human immunodeficiency virus (HIV) and determined to be unreactive for hepatitis B and C and unreactive for human immunodeficiency virus (HIV) by ELISA. However, products of human origin should be considered potentially infectious and handled accordingly.
Notes
It is not unusual for our human serum products to be cloudy. This product comes from pooled serum and turbidity indicates that lipids are present in the serum. Clear, lipid-free human serum products are not available.
Which document (s) contains information about the shelf life or expiration date of a given product?
If available for a particular product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
There is a precipitate in my serum. Is my serum contaminated?
Serum precipitates can often appear as large round bodies, even doublets on microscopic examination. This can be mistaken for yeast contamination. Brownian motion can be confused with motility. These are aggregates of lipids that will dissolve easily when exposed to lipase. Furthermore, these particles will not stain Gram or show any growth in culture under aerobic, anaerobic, or fungal culture media and conditions. Material performance is not affected. Small volume centrifugation or filtration will remove these particles.
How do I obtain lot-specific information or a certificate of analysis?
The lot-specific COA document can be found by entering the lot number above in the “Documents” section.
Product H4522, Human Serum, Heat Inactivated (HI)?
This serum is not heat-inactivated. If heat-inactivated serum is required, the serum can be heated to 56 ° C for 30 minutes. Heating inactivates the complement cascade. Active complement can participate in cytolytic events, leading to smooth muscle contraction, histamine release from mast cells and platelets, and activation of lymphocytes and macrophages.
How is the product H4522, human serum prepared?
Blood is collected and citrate is added as an anticoagulant to inhibit clotting. The blood is then centrifuged and separated into cells and plasma. Plasma is separated from cells and calcium is added to the plasma to activate the clotting cascade. Then the preparation is centrifuged again and the serum is the remaining liquid product. This process produces a serum low in growth factors since it does not contain the factors released by cells when clotting.
Product H4522, human serum, is cloudy. What Causes Cloudiness?
It is not unusual for our human serum products to be cloudy. This product comes from pooled serum and turbidity indicates that lipids are present in the serum. Clear, lipid-free human serum products are not available.
Why do we specify male AB in the description of Product H4522, Human Serum?
Male blood is used in serum products, when specified, because female serum may have more antibodies as a result of pregnancy. Type AB serum is used as there are no anti-A and/or anti-B antibodies found in other blood types.
How do I find price and availability?
There are several ways to find prices and availability for our products. Once you log in to our website, you will find the price and availability shown on the product detail page.